ABSTRACT
Scolopendra polymorpha (S. polymorpha) is a predatory centipede whose venom contains a multiplicity of biochemical effectors that can cause muscle damage and cumulative cell destruction in its prey. Despite previous investigations of S. polymorpha and other centipede venoms, there is a lack of information on the morphological and biochemical patterns elicited by their myotoxic effects. To elucidate these processes, this paper presents evidence of skeletal muscle damage, and alterations in key biochemical mediators that appear only after exposure to centipede venom. Methods: Venom was collected and fractionated using RP-HPLC; mouse extensor digitorum longus (EDL) muscle was exposed to whole venom and venom fractions to evaluate myotoxicity by means of creatine kinase (CK) - a muscle damage marker - activity measurements and histochemical analysis. Results: CK activity was higher in EDL muscle exposed to venom than in unexposed muscle. This increase was observed after 15 min of venom incubation, and remained stable up to 45 min. Venom-exposed EDL muscle showed signs of muscle damage including necrosis, loss of fascicular structure as well as mitochondrial accumulations and ragged red fibers (RRF), suggesting an impairment in the normal mitochondrial arrangement. Nicotinamide adenine dinucleotide (NADH) and cytochrome oxidase (COX) tests also indicate that respiratory complexes might be affected. Conclusion: Our results suggest a different biochemical composition of S. polymorpha venom, based on the different effects of four venom fractions on the cells tested, according to statistical evidence. Fractions F6 and F7 caused the most important alterations.(AU)
Subject(s)
Animals , Mice , Creatine Kinase , Myotoxicity , Chilopoda , Biochemistry , Chromatography, High Pressure LiquidABSTRACT
The main objective of this study was to validate and examine the psychometric parameters of Profil der Lebensqualität Chronischkranker (PLC) in climacteric women. We conducted an exploratory research in Health Care and Treatment enrollment 280 participants. The data collection instruments were the PLC and the SF- 36 (Medical Outcomes Study 36 - Item Short - Form Health Survey). The original German version of PLC consists of 40 items that explore the biological and psychosocial items of daily life. The questionnaire was submitted to translation into Portuguese of Brazil, back translation, review by experts and cultural adaptation. For the psychometric study, Cronbach's alpha, factor analysis and the Pearson correlation coefficient were used.In the internal consistency analysis, Cronbach's alpha coefficient ranged from 0.81 a 0.89 in all categories, indicating that the instrument has adequate internal consistency. We could verify the existence of five factors that together explained 55.85% of total variance. For criterion validity, Pearson's correlation was between 0.41 and 0.78. Therefore, the Brazilian version of the PLC is configured in valid, reliable and easy-to-use option for assessment of quality of life in climacteric women.
O objetivo deste estudo foi validar e verificar os parâmetros psicométricos da Escala Profil der Lebensqualität Chronischkranker (PLC) para uma amostra de 280 mulheres climatéricas. Realizou-se uma pesquisa exploratória, cujos instrumentos de coleta foram o PLC e o SF-36 (Medical Outcomes Study 36 - Item Short - Form Health Survey). A versão original em alemão do PLC compõe- se de 40 itens que exploram os itens biológicos e psicossociais da vida diária. Foi realizada a tradução para o português do Brasil, retrotradução, revisão por painel de especialistas e adaptação cultural. Para o estudo psicométrico foram utilizados alfa de Cronbach, análise fatorial e coeficiente de Pearson. O coeficiente alfa de Cronbach variou de 0,81 a 0,89 em todas as categorias, apontando que o instrumento apresenta adequada consistência interna. Quanto à validade de constructo, verificou- se a existência de cinco fatores (estado de ânimo negativo, capacidade física, função social, estado de ânimo positivo e bem- estar social) que juntos explicam 55,85 % da variância total. Para validade de critério, os valores da correlação de Pearson situaram -se entre 0,41 e 0,78. Sendo assim, a versão brasileira do PLC configura-se em opção válida, confiável e de fácil aplicação para avaliação da qualidade de vida em mulheres climatéricas.
Subject(s)
Humans , Female , Quality of Life , Climacteric , Cross-Sectional Studies , Validation StudyABSTRACT
Se realiza una puesta al día en aspectos histopatológicos de los linfomas no-Hodgkin en pediatría. El interés de esta comunicación es poner a disposición del pediatra los aspectos prácticos de estas entidades desde el punto de vista de la anatomía patológica con especial referencia al empleo de la inmunotipificación